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KMID : 1123920150290060475
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Korean Journal of Oriental Physiology and Pathology
2015 Volume.29 No. 6 p.475 ~ p.484
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Antioxidant and Protective Effects of Gagam-Danguieumja on Ultraviolet B-induced Damage in Human Keratinocytes
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Kim Tae-Yeon
Park Jong-Phil
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Abstract
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In this paper, we studied the anti-oxidative capacities and protective effects of water extract of Gagam-Danguieumja(GDE) against Ultraviolet B(UVB)-induced oxidative damage in human keratinocytes(HaCaT). To evaluate the anti-oxidative activities of GDE, we measured scavenging activities on DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion, lipid peroxidation and reducing power of GDE. To detect the protective effects of GDE against UVB, we irradiated with 40 mJ/㎠`s UVB to HaCaT cells then we measured reactive oxygen species(ROS) generation, apoptotic bodies and cell viability using DCFH-DA assay, Hoechst 33342 staining and MTT assay. GDE showed the anti-oxidative activities by scavenging DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion, lipid peroxidation. Also GDE showed high reducing values. GDE reduced oxidative stress conditions by inhibition of ROS expression. Also the cell apoptosis by UVB-induced oxidative conditions was decreased by GDE treatment. These results could suggest that GDE had anti-oxidative activities and exhibited protective effects against UVB on HaCaT cells. GDE would be useful for the development of cosmetics treating UVB-induced skin aging.
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KEYWORD
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Gagam-danguieumja(GDE), Ultraviolet B(UVB), Human keratinocytes(HaCaT), Anti-oxidative activity, Apoptosis, Reactive oxygen species(ROS)
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